HIV infection is a risk factor for liver fat accumulation

Hugo Perazzo at AIDS 2018. Photo by Liz Highleyman.

People living with HIV are about twice as likely to develop steatosis, or fat accumulation in the liver, even if they don't have hepatitis B or C co-infection, according to a study presented at the recent 22nd International AIDS Conference (AIDS 2018) in Amsterdam. 

Liver disease is a major cause of morbidity and mortality among people living with HIV. Hepatitis B or C virus (HBV or HCV), heavy alcohol consumption, certain antiretroviral medications and other causes of liver injury can lead to the development of liver fibrosis, cirrhosis and steatosis. But these can also occur without such liver-specific risk factors. Previous studies have seen steatosis rates among people with HIV ranging from around 35% to 75%.

Fatty liver disease, associated with metabolic syndrome, is a growing problem as the population becomes more obese. Over time, non-alcoholic fatty liver disease (NAFLD) and its more advanced stage, non-alcoholic steatohepatitis (NASH), can lead to liver function impairment, liver cancer and the need for a liver transplant.

Glossary

body mass index (BMI)

Body mass index, or BMI, is a measure of body size. It combines a person's weight with their height. The BMI gives an idea of whether a person has the correct weight for their height. Many BMI calculators can be found on the internet.

metabolism

The physical and chemical reactions that produce energy for the body. Metabolism also refers to the breakdown of drugs or other substances within the body, which may occur during digestion or elimination.

non-alcoholic fatty liver disease (NAFLD)

Non-alcoholic fatty liver disease (NAFLD) is a very common disorder and refers to a group of conditions where there is accumulation of excess fat in the liver of people who drink little or no alcohol. The most common form of NAFLD is a non-serious condition called fatty liver, by which fat accumulates in the liver cells. A small group of people with NAFLD may have a more serious condition named non-alcoholic steatohepatitis (NASH).

lipid

Fat or fat-like substances found in the blood and body tissues. Lipids serve as building blocks for cells and as a source of energy for the body. Cholesterol and triglycerides are types of lipids.

syndrome

A group of symptoms and diseases that together are characteristic of a specific condition. AIDS is the characteristic syndrome of HIV.

 

Hugo Perazzo of Fundação Oswaldo Cruz in Rio de Janeiro and colleagues evaluated the prevalence of and risk factors associated with liver steatosis in people with HIV mono-infection –  that is, those without HBV or HCV co-infection – compared with HIV-negative individuals.

The study drew from 649 people with HIV followed at the National Institute of Infectology (INI/Fiocruz) and 15,105 HIV-negative people in the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil) cohort.

In the HIV-positive group 58% were men, 23% were black or 'Pardo' (mixed race) and the median age was 44 years. The median body mass index (BMI) was 24.4 (25 and above is considered overweight) and a third had metabolic syndrome, a cluster of cardiovascular risk factors including abdominal obesity, hypertension (high blood pressure), high blood glucose and abnormal lipid levels.

A total of 333 HIV-positive participants were paired with an equal number of HIV-negative people. Although they were approximately matched based on demographic characteristics and metabolic factors, there were some significant differences. The HIV-positive group was younger, had fewer women, were less likely to be black/Pardo and were more likely to have diabetes, while the HIV-negative group had higher average BMI and were more likely to have abnormal lipids, hypertension and metabolic syndrome.

For each participant, the researchers calculated a Fatty Liver Index (FLI) score based on laboratory and physical exam findings including triglyceride and gamma-glutamyl-transferase (GGT) liver enzyme levels, BMI and waist circumference. Liver steatosis was defined as an FLI of 60 or higher.

More than a third of the participants with HIV (35%) were found to have steatosis. HIV infection was an independent risk factor, doubling the likelihood of having fatty liver, Perazzo reported.

HIV-positive people with steatosis were, on average, older, had higher BMI and waist circumference, had a higher CD4 count and had been on antiretroviral therapy (ART) longer than those without. There were no significant differences related to nadir (lowest-ever) CD4 count, current ART regimen or current viral suppression.

About two-thirds (68%) of the HIV-positive people with steatosis had metabolic syndrome, 46% had abnormal lipids, 46% had hypertension, 41% had diabetes and 54% were described as having 'poor clinical management'.

In a multivariate analysis, independent risk factors for fatty liver were HIV infection (odds ratio 2.1), male sex (OR 5.36), being black/Pardo (OR 0.22), higher BMI (OR 1.91), diabetes (OR 5.79), abnormal lipids (OR 2.57) and hypertension (OR 2.56). In addition, higher CD4 count (OR 1.13 per 100 cell/mm3), higher cumulative HIV viral load (OR 1.25 per 10 log copies/ml per year) and poor clinical management (OR 0.36) also increased the risk of steatosis.

"HIV-infected individuals had two-fold higher odds for presence of steatosis compared to uninfected paired controls," the researchers concluded. "Traditional and HIV-specific risk factors were independently associated with liver steatosis in people living with HIV."

They recommended that management of metabolic risk factors should be integrated into HIV care to decrease the burden of liver diseases in this population.

In response to a question, Perazzo said that FibroScan, an imaging method for assessing fibrosis and steatosis, was not available at the time of the study. Session moderator Marina Klein of McGill University in Montreal noted that researchers are looking for more accurate non-invasive biomarkers of fatty liver as it becomes a more prevalent cause of serious liver disease in people with and without HIV.

References

Perazzo H et al. HIV infection is an independent risk factor for liver steatosis: A study in HIV mono-infected patients compared to uninfected paired controls and associated risk factors. 22nd International AIDS Conference, Amsterdam, abstract THAB0205, 2018. 

View the abstract on the conference website.

Download the slides from the conference website.

Watch the webcast of this session on YouTube.