HPTN 052: early treatment reduces serious illness by 40%

Keith Alcorn
Published: 18 July 2011
Beatriz Grinsztejn and Mina Hossienipour ©IAS/Marcus Rose/Worker's Photos

Early treatment in the HPTN 052 study of treatment as prevention reduced serious illness by around 40%, delegates at the Sixth International AIDS Society conference (IAS 2011) heard – but the effect was almost entirely accounted for by fewer cases of extrapulmonary tuberculosis.

There was no significant difference in deaths, or in the rate of serious bacterial infections or of pulmonary tuberculosis.

The HPTN 052 study was designed to determine whether early treatment for an HIV-infected person would reduce the risk of HIV transmission to his or her regular partner.

The study showed that early antiretroviral treatment – started at a CD4 count between 350 and 550 cells/mm3 – reduced the risk of HIV transmission to uninfected partners by at least 96%.

However, the study also collected information on a number of other endpoints: death, illness , virological and immunological responses to treatment. These data will provide important information on the potential benefits of early treatment in settings where bacterial infections and tuberculosis are more prevalent than in North America and Europe.

The study will also provide information on any differences in rates of clinical or immunological disease progression between different regions of the world, or between men and women. Some studies, but not all, have suggested faster rates of disease progression in sub-Saharan Africa.

The study enrolled 1763 HIV-infected individuals with CD4 counts between 350 and 550 cells/mm3. Participants were randomised either to receive immediate antiretroviral treatment or to defer treatment until their CD4 cell count fell below 250 cells/mm3 on two separate tests. This was the level at which treatment was recommended to begin in national guidelines during the study recruitment period. The study recruited participants in Malawi, India, Zimbabwe, Botswana, South Africa, Kenya, Thailand, the US and Brazil.

The median CD4 count at enrolment was 446 cells/mm3 and the median viral load 4.4 log10 copies/ml (25,000 copies/ml).

Although the proportion of HIV-positive men and women recruited to the study was equal across the study population as a whole, sites in Africa recruited a larger proportion of women than men (58 vs 40%, p< 0.0001), reflecting the higher rates of engagement in care by women in sub-Saharan Africa as a consequence of earlier HIV diagnosis through antenatal programmes.

African sites also recruited a larger proportion of participants in the 18 to 24 age group (20 vs 15%, p=0.003). African participants had slightly lower viral loads (4.4 vs 4.5 log10 HIVRNA, p=0.006).

Although there was no significant difference in baseline CD4 count between regions, Africans in the deferred treatment arm were less likely to reach the CD4 threshold that indicated they needed to start treatment. Participants in the deferred arm outside the Africa region were 40% more likely to start treatment, but when the hazard ratio was adjusted for confounding factors this difference ceased to be statistically significant (aHR 1.30, 95% CI 0.9-1.8, p= 0.06).

The median time to starting treatment in the deferred arm was 3.25 years outside Africa, compared to 4.1 years in Africa.

Overall, 21% of participants in the deferred arm needed to start treatment during the follow-up period (n=184), an incidence rate of 12 per 100 person-years of follow-up. However, among Africans the rate of treatment initiation was 9 per 100 person-years, compared to 15 per 100 person-years of follow-up in participants at non-African sites. 

Unsurprisingly, the factors significantly associated with the need to start treatment in the deferred treatment arm were lower CD4 count or higher viral load at enrolment (adjusted hazard ratio per increase of 100 CD4 0.6; 95% CI,0.5-0.7) and higher log10 HIV RNA (adjusted hazard ratio per log increase 1.5; 95% CI, 1.2-1.8).

Overall, 75% of patients in the deferred arm who initiated treatment did so because of a decline in CD4 cell count.

Among those who initiated treatment immediately (886 patients), at a median CD4 count of 442 cells/mm3, 90% had an undetectable viral load (below 400 copies/ml) after one year of treatment. They experienced a mean CD4 cell increase of 158 cells/mm3, to 603 cells/mm3.

In the delayed-treatment group, the median CD4 count at treatment initiation was 225 cells/mm3, and the median time to treatment initiation in the deferred arm was 3.5 years. After one year, 93% had an undetectable viral load (below 400 copies/ml) and CD4 cell counts had risen by a mean of 191 cells/mm3, to 418 cells/mm3.

Virologic failure was rare; only 5% in the immediate-treatment arm and 2.7% in the deferred-treatment arm experienced virologic failure. Of these, 67% in the immediate arm and 60% in the deferred arm switched to second-line therapy.

The effect of early treatment on clinical outcomes was very clear.

Early treatment significantly reduced the risk of clinical illness, but there was no difference in the risk of death between the two study arms.

Primary endpoint clinical events were defined as:

  • A WHO stage IV event.
  • Pulmonary tuberculosis.
  • Severe bacterial infection.
  • Death.

Around 7% of participants received cotrimoxazole prophylaxis against bacterial infections, and around 4% received isoniazid preventive therapy to prevent the development of active TB.

A total of 105 participants developed a primary clinical event during 3304 person-years of follow-up. Forty primary events occurred in the immediate arm (2.4 per 100 PY), compared to 65 in the deferred arm (4.0 per 100 PY, hazard ratio 0.59, 95% CI: 0.40 - 0.88, p=0.01.)

Early treatment did not significantly reduce the risk of developing pulmonary tuberculosis. There were 14 cases of pulmonary TB in the immediate-treatment arm (0.8 per 100 PY), compared with 16 in the delayed-treatment arm (0.9 per 100 PY). Pulmonary TB cases were diagnosed at a median CD4 count of 521 in the immediate-treatment arm and 295 in the delayed-treatment arm.

In contrast, early treatment did significantly reduce the risk of extrapulmonary TB. Three cases were diagnosed in the immediate-treatment arm (0.2 per 100 PY), at a median CD4 count of 443, compared to 17 in the delayed-treatment arm (1 per 100 PY), at a median CD4 count of 342.

Bacterial infections occurred somewhat more frequently in the immediate-treatment arm (19 vs 13, 1.1 vs 0.8 per 100 PY). In each arm the most common bacterial infection was pneumonia, and the difference between arms was chiefly driven by four cases of sepsis in the immediate-treatment arm. Three participants in the immediate arm each experienced more than one bacterial infection.

There was no significant difference in the death rate between arms: ten occurred in the immediate arm and 13 in the deferred arm. Deaths were largely attributed to causes other than HIV in the immediate arm: 3 suicides, one stroke, and three unknown causes, with only three deaths due to infection (leptospirosis, TB and sepsis) in the immediate arm. Almost half the deaths in the delayed arm were of unknown cause, with two accidental deaths, one stroke and only two deaths due to infection.

There was no significant difference in severe adverse events between the two arms (14% in each), nor any difference in the distribution of types of adverse event between the two arms.

References

Grinsztejn B et al. Effects of early versus delayed initiation of antiretroviral therapy (ART) on HIV clinical outcomes: results from the HPTN 052 randomised clinical trial. Sixth International AIDS Society Conference on HIV Pathogenesis, Treatment and Prevention, Rome, abstract MOAX0105, 2011.

Hosseinipour MC et al. Immunologic and virologic disease progression and responses to ART across geographic regions: outcomes from HPTN 052 study. Sixth International AIDS Society Conference on HIV Pathogenesis, Treatment and Prevention, Rome, abstract MOAX0104, 2011.

View abstract MOAX0104 on the conference website

View the presentation by Mina Hosseinipour, with audio, on the conference website

View abstract MOAX0105 on the conference website

Download the powerpoint presentations for the HPTN 052 session from the conference website

This report is also available in French.