Considerations

The tests are expensive and technically complex to perform.

A reactive result – especially with a low viral load – should be interpreted with caution and probably not viewed as positive unless it is confirmed on a subsequent test. Results can be confirmed by a p24 antigen test, polymerase chain reaction (PCR) for DNA or cell culture. It may be necessary to wait a few days before another test can give a reliable result.

It may be appropriate to use this method:

  • To identify people who are infected but have not seroconverted.
  • To test babies.
  • As a confirmatory test, especially if the results of other tests are indeterminate.